Studies investigating a specific sub-clone and cultivation parameters can easily become an extensive task. DASGIP offers a parallel small-scale cultivation solution providing tightly controlled conditions that integrate separate modules for monitoring, dosing, feeding and documentation
Samples can be taken from one of up to 18 ports of the reactors' sterile head plates. Individual parameters of a single or full culture can be isolated and optimized.The DASGIP system was utilised in a study into the impact of a small-scale, stirred cultivation system with oxygen and pH control, using a CHO cell line secreting the recombinant protein (MUC1-Fc). The system included Micro-Pumps, Gas-Mixing Station and ph- and DO control, all integrated into the DASGIP monitoring, documentation and steering system. The DASGIP system was found to provide:
Reduced working volumes under controlled conditions. Similar mixing and oxygen transfer characteristics to stirred systems of a greater volume, resulting in good scalability. Suitable results for different mammalian cell culture applications such as process development, cellular engineering and primary cell application.
